知识与财富的链接
与opaque-2（o2）基因紧密连锁的分子标记,可以有效地用于优质蛋白玉米育种。本研究利用SSR标记,检测了QPM群体中群13、中群14内o2基因的频率分布,发现中群13、中群14在o2基因位点基因频率发生了改变。检测了从QPM群体中选育的高代系中o2基因的频率,发现在CA、CD、CB和R高代系中o2o2基因型频率分别为91.7%、80.0% 、0.0和100%。表明用常规方法选育QPM自交系,不能有效地保证o2基因纯合。为了拓宽QPM种质基础,利用分子标记追踪普通玉米与QPM自交系回交后代的o2基因,将普通自交系转育为QPM,结果表明非常有效。
The SSR markers linked tightly with opaque-2 (o2) can be used effectively in the quality protein maize (QPM) breeding.In this study,the SSR primer phi057 was used in the evaluation of inbred lines developed in recent years,and QPM breeding program to convert the elite normal lines into QPM.Mutation on o2 was found during the improvement of the QPM populations CPop13 and CPop14 after detection of o2 gene frequency with the marker.The o2o2 genotypic frequency in CA,CD,CB,and R series derived from the improved populations was detected as 91.7%,80.0%,0.0,and 100%,respectively.It was indicated that homozygous o2o2 genotype in QPM could not be ensured in conventional breeding efforts.In order to enhance the QPM germplasm basis and convert elite normal lines into QPM,the markers were used to detect o2 gene in backcross generations,and it has proved that it is a powerful tool in the breeding.
