为明确土壤养分含量及化学计量比对多种限制性养分添加的响应,分析土壤中速效养分与全量养分相关关系,以及土壤全量和速效化学计量特征的指示作用,阐明养分添加对荒漠草原土壤养分动态平衡关系的影响.以宁夏荒漠草原为研究对象,于2018年开始进行氮(N)和磷(P)养分添加控制试验,试验处理包括对照(CK)、N添加、P添加和NP共同添加这4个处理.结果表明:(1)养分添加第4年,土壤N含量显著增加;N添加显著增加土壤N∶P, P添加和NP共同添加显著增加土壤有机碳(SOC)含量.养分添加第3年和第4年,N添加显著提高土壤速效N∶P(AN∶AP);N添加和NP共同添加显著增加土壤铵态氮(NH+4-N)和硝态氮(NO-3-N)含量,而降低土壤速效C∶N(C∶AN);P添加和NP共同添加显著增加土壤全磷(TP)和速效磷(AP)含量,而降低土壤全量和速效N∶P、C∶P.(2)N添加和P添加对荒漠草原土壤NH+4-N、AP含量和土壤速效C∶N、AN∶AP的影响具有显著交互作用.(...
In order to discuss the response of soil nutrient content, stoichiometric ratio, and dynamic nutrient balance to the addition of multiple restrictive nutrients, the correlation between available nutrients and total nutrients in soil, as well as the indication of soil total and available stoichiometric characteristics, were studied in a desert grassland subjected to 4 years of nutrient addition treatments. The Ningxia desert grassland was used as the research object to carry out nitrogen (N) and phosphorus (P) addition experiments. The experiment included four treatments:control (CK), N addition[10 g·(m2·a)-1], P addition[10 g·(m2·a)-1], and NP co-addition (10 g·(m2·a)-1 N+10 g·(m2·a)-1 P). The results showed that:① in the fourth year of nutrient addition, soil total nitrogen (TN) content was significantly increased. The N:P ratio was significantly increased by N addition, and soil organic carbon (SOC) content was significantly increased by P addition and NP co-addition. In the third and fourth years of nutrient addition, the soil available N:P ratio (AN:AP) was significantly increased by N addition; N addition and NP co-addition significantly increased the content of ammonium nitrogen (NH4+-N) and nitrate nitrogen (NO3--N) but significantly reduced the soil available C:N ratio. P addition and NP co-addition significantly increased total phosphorus (TP) and soil available phosphorus (AP), whereas it significantly reduced the soil total and available C:P and N:P ratios. ② The interaction between N addition and P addition had a combined effect on NH4+-N, AP, available C:N, and AN:AP ratio of desert grassland. ③ The soil C:N ratio was relatively stable in desert grassland, soil N:P ratio was mainly limited by soil TP content, and the soil available C:P and AN:AP ratios were mainly limited by soil AP content. ④ There were cumulative effects of N and P additions on soil N, SOC, and inorganic nitrogen. N limitation in desert grassland was alleviated by N addition, whereas it was aggravated by P addition and NP co-addition. The variation coefficients of soil available stoichiometric characteristics were higher than that of soil total stoichiometric characteristics. Soil available stoichiometry was more sensitive to N and P addition than soil total stoichiometry in desert grassland, which could better reflect the effects of N and P addition on soil ecological stoichiometry and as a rapid indicator of soil nutrient status in desert grassland.