This study aimed to clone the full-length cDNA of the porcine ADAR2 gene and explore its expression pattern in different tissues of pigs.Rapid-amplification of cDNA ends(RACE)was used to clone the full-length cDNA sequence of the ADAR2 gene in Large White pigs and the sequence was analyzed by bioinformatics.Real-time PCR was used to detect the ADAR2 mRNA expression in the heart,liver,lung,kidney,spleen,brain,small intestine,muscle and backfat of 35-day-old pigs.Porcine ADAR2 cDNA sequence of 6305 bp was cloned,which contained 12 exons and encoded 704 amino acids.The nucleic acid and amino acid sequences shared a high identity(>84%)with that of other mammals including human,chimpanzee,macaque,gibbon,cow,goat and sheep.The deduced ADAR2 had two double-stranded RNA binding motifs and an adenosine deaminase domain.Real-time PCR results showed that the ADAR2 expressed in all the detected tissues,and had the highest expression in the lung.The full-length cDNA sequence of porcine ADAR2 gene was successfully cloned,and it was widely expressed in tissues of pigs.These findings provide a solid foundation for further function study of ADAR2.