Objective To construct Tujia medicine Tianzhusan added medicine prescription and explored its effectiveness in anti stroke. Methods Focusing on stroke, firstly, the scope of candidate herbals was determined by regional questionnaire survey, and network pharmacology was used to analyze the candidate herbals and disease targets to determine the best new prescription. Then, according to the compatibility theory "Zhu Bang Ke Xin" of Tujia doctors, the new prescription was rationally evaluated. Molecular docking technology was used to explore the mechanism of the new prescription in the treatment of stroke preliminarily.Results Among the herbals recommended by Tujia doctors for the treatment of stroke with Tianzhusan, Bajiaofeng (Alangium Chinense), Sanjiaofeng (Hedera Nepalensis) and Touyuncao (Geum Japonicum Thunb) were top ranked. By comparing the topological parameters of each candidate herbal prediction targets and disease targets network, it was determined that Tianzhusan plus Touyuncao (Geum Japonicum Thunb) was the best compatibility of the new prescription, in which Tianma (Gastrodiae Rhizoma) was the “Zhu” herbal, Yanlingcao (Trillium Tschonoskii) was the “Bang” herbal, and Touyuncao (Geum Japonicum Thunb) might act as the “Bang”, “Ke” and “Xin” herbal at the same time. The new prescription might intervene the symptoms of stroke by participating in the regulation of nitric oxide biosynthesis, the positive regulation of phosphorylation, the response to oxygenates, the maintenance of neuronal projection and other biological processes. The results of molecular docking showed that the constituents of 26-chloro-26-deoxycryptogenin, β-ecdysterone, ecdysterone, gastrodin and 4-Hydroxycinnamic acid had good binding activities with proteins APP, IL6, mTOR, JAK2 and TLR4 respectively. Conclusion Tianzhusan combined with Touyuncao (Geum Japonicum Thunb) is reasonable and effective, which may increase the effect of the original prescription Tianzhusan in the treatment of stroke.