知识与财富的链接
【目的】测定和分析半翅目(Hemiptera)仁蚧科(Aclerdidae)首个线粒体全基因组——高桥仁蚧Aclerda takahashii线粒体全基因组序列,并探讨与其他蚧虫类群的系统发育关系。【方法】基于Illumina测序技术进行高桥仁蚧线粒体全基因组测序,并进行生物信息学分析;基于已报道的15科31种半翅目昆虫的线粒体全基因组序列运用最大似然法(ML)和贝叶斯推断法(BI)构建半翅目系统发育树。【结果】高桥仁蚧线粒体基因组全长16 599 bp, AT含量高达84.51%。在该线粒体基因组中,缩减tRNA非常普遍, 10个tRNA缺失二氢尿嘧啶(DHU)臂或TΨC臂, tRNAser(S1)和tRNAser(S2)缺失DHU臂和TΨC臂。系统发育树显示, 与仁蚧科亲缘关系最近的是蚧科(Coccidae)。【结论】本研究报道了首个仁蚧科的线粒体基因组, 发现在高桥仁蚧线粒体基因组中存在普遍的tRNA缺臂现象, 为进一步系统地研究蚧虫线粒体基因组提供了数据支持。
【Aim】 This study aims to sequence and analyze the complete mitochondrial genome (mitogenome) of Aclerda takahashii, which represents the first complete mitogenome of the family Aclerdidae of Hemiptera, and to investigate the phylogenetic relationships with other Coccoidea groups. 【Methods】 The whole mitogenome of A. takahashii was sequenced based on the Illumina sequencing technique, and analyzed with bioinformatics methods. The phylogenetic trees of hemipteran insects were constructed based on the reported complete mitochondrial genomes of 31 speciec of 15 families of Hemiptera using the maximum likelihood (ML) and Bayesian inference (BI) methods. 【Results】 The mitogenome of A. takahashii is 16 599 bp in length with high A+T content (84.51%). The truncated tRNAs are common in this mitogenome, ten tRNAs lacking of a dihydrouridine (DHU) arm or TΨC (T) arm. The DHU and T arm of tRNAser(S1) and tRNAser(S2) are both missing. The phylogenic trees showed that Aclerdidae has the closest relationship with Coccidae. 【Conclusion】 This study reported the first mitogenome of Aclerdidae, and revealed that truncated tRNAs are prevalent in the mitogenome of A. takahashii, providing data for the further systematic research of mitogenomes of scale insects.
