Objective To detect and analyze the in vitro metabolites of AB-CHMINACA which were generated in the in vitro human liver microsomes model system by Ultra Performance Liquid Chromatography(UPLC)coupled to High Resolution Mass Spectrum(HR-MS).And then compare the results with the urine sample with a real abuser.Methods To simulate the metabolic process in human body by establishing the in vitro human liver microsomes model,and then determine and elaborate the reaction points and pathways of metabolism by HR-MS.Results AB-CHMINACA generated 10 metabolites in the in vitro human liver microsomes model totally.Carboxamide hydrolysis,hydroxylation on the methylpropyl moiety and the combination of N-dealkylation and carboxamide hydrolysis were its main metabolic pathways.Carboxamide hydrolysis metabolite M1,hydroxylation on the methylpropyl moiety metabolite M4 and the combination of N-dealkylation and carboxamide hydrolysis metabolite M10 also appeared in the urine sample of a AB-CHMINACA abuser.Conclusion The method is fast and efficient,and it can predict the in vivo metabolites to a certain extent.So we suggest that the carboxamide hydrolysis metabolite and hydroxylation on the methylpropyl moiety metabolite can be used as biomarkers for routine analysis of urine samples.