知识与财富的链接
[目的]探讨LncRNA ESCCAL＿1对食管鳞状细胞癌免疫原性细胞死亡的影响。[方法]分别将食管鳞状细胞癌细胞株KYSE450、KYSE70分为shNC组和shESCCAL＿1组。采用慢病毒介导的RNA干扰技术,敲减KYSE450、KYSE70细胞LncRNA ESCCAL＿1后,采用流式细胞术检测钙网蛋白和细胞凋亡;酶联免疫吸附实验（ELISA）检测细胞上清中高迁移率族蛋白1(HMGB1)及ATP含量。敲减ESCCAL＿1的KYSE450、KYSE70细胞与DC细胞共培养后,流式细胞术检测DC细胞表面标记CD86和CD1a的表达。[结果] KYSE450、KYSE70细胞敲减ESCCAL＿1后,与shNC相比,shESCCAL＿1组细胞钙网蛋白阳性表达均明显升高（t=21.96、31.11,P均<0.001）,KYSE450和KYSE70细胞中shESCCAL＿1组细胞凋亡增加（t=18.37、5.16,P均<0.01）。在KYSE450和KYSE70细胞中,与shNC组相比,敲减ESCCAL＿1组ATP释放明显增加（t=23.24、23.86,P均<0.01）...
Abstract： ［Objective］ To investigate the effect of lncRNA ESCCAL_1 on immunogenic cell death in esophageal squamous cell carcinoma. ［Methods］ Human esophageal squamous cell carcinoma(ESCC) KYSE450 and KYSE70 cells were transfected with shNC or shESCCAL_1. After knocking down of ESCCAL_1 expression, the expression of cellular calreticulin(CRT) and cell apoptosis were detected by flow cytometry, the contents of high mobility group box 1(HMGB1) and ATP in cell culture supernatant were measured by ELISA method. After co-culture with transfected KYSE450 and KYSE70 cells, the expression of CD1a and CD86 in dendritic cells(DC) was detected by flow cytometry. ［Results］ After knocking down of lncRNA ESCCAL_1 in KYSE450 and KYSE70 cells, the cell surface expression of CRT in shESCCAL_1 group was significantly higher than in that in shNC group（t=21.96 and 31.11, all P＜0.001）, cell apoptosis was significantly increased(t=18.37 and 5.16, all P＜0.01）. Compared with shNC groups, the contents of ATP and HMGB-1 in shESCCAL_1 group were increased significantly（t=23.24 and 23.86, all P＜0.01; t=48.69 and 49.97, all P＜0.001, respectively）. Compared with shNC groups, the expression of CD86 and CD1a in DC cells co-cultured with shESCCAL_1-transfected KYSE450 and KYSE70 cells was significantly increased（t=45.88 and 29.45, all P＜0.001; t=32.45 and 37.41, all P＜0.001, respectively）. ［Conclusion］ Down-regulated expression of lncRNA ESCCAL_1 promotes immunogenic cell death in esophageal squamous cell carcinoma cells, which indicates that lncRNA ESCCAL_1 may affect tumor immunity.
