Arachis hypogaea L. is one of the most important oilseed and cash crops. The nitrogen utilization efficiency (NUE) is an important factor of peanut yield. In previous study, a candidate gene related to peanut yield was identified, belonging to high-affinity nitrate transporter genes (NRT2) family. In this project, the conserved domain of NRT2 protein was used to obtain the members of the NRT2 gene family based on the whole genome and transcriptome of peanut database. The phylogenetic tree, physicochemical properties, chromosomal distribution, genetic structure, conserved domain and gene expression pattern from 22 tissues were analyzed. The results showed that a total of 10 family members of the peanut NRT2 gene were identified. Chromosomal location analysis showed that these members were unevenly distributed on the 20 chromosomes.
Genes located on chromosome 03 and chromosome 13, chromosome 06 and chromosome 16 had significant homology relationships. The expression pattern analysis indicated that the expression levels in tissues were different. Four genes, AhNRT2.4,AhNRT2.5a,AhNRT2.5b and AhNRT2.5c had higher expression in the root, which indicated the NRT2 members played important roles in root functions. This study provided the theoretical evidence for further revealing the function of NRT2 protein in peanut.