知识与财富的链接
目的 人参作为一种传统中药,其含有抗氧化、抗炎、抗衰老等多种活性物质。许多研究报道其具有预防与治疗糖尿病的功能。但其是否具有促进胰岛β细胞再生的功能还未研究。本文利用斑马鱼作为实验动物,来研究人参发酵萃取物与胰岛β细胞再生的关系。方法 本文通过胰岛特异性标记的系列转基因斑马鱼模型,激光共聚焦显微镜成像等方法来评估人参发酵萃取物能否促进胰岛β细胞再生,以及促β细胞再生的作用方式。结果 人参发酵萃取物能够显著促进糖尿病斑马鱼模型中的β细胞再生,并降低血糖。进一步发现其作用方式是通过促进胰腺内分泌前体细胞向β细胞分化,从而促进β细胞再生。结论 人参发酵萃取物具有促斑马鱼胰岛β细胞再生的作用。而这种促胰岛β细胞再生的作用主要是通过促进胰腺内分泌前体细胞向胰岛β细胞分化达成的。
Objective Ginseng is a traditional Chinese herbal medicine, contains various bioactive compounds with antioxidant, anti-inflammatory, and anti-aging properties. Studies have reported that ginseng has preventive and therapeutic effects on diabetes. However, whether extract from ginseng has activity to promote pancreatic β-cell regeneration remains unclear. In this study, we investigated the relationship between fermented ginseng extract (FGE) and β-cell regeneration using zebrafish as a model.Methods We employed a series of transgenic zebrafish models, which endocrine cell labelled with florescence proteins to evaluate whether FGE could enhance β-cell regeneration. Moreover, utilized confocal imaging and other techniques to assess the detail action underlying FGE’s promotion of β-cell regeneration.Results FGE significantly promoted β-cell regeneration in a zebrafish model of diabetes and lowed blood glucose levels. Further studies revealed that FGE promote β-cell regeneration due to increasing of endocrine precursor cell differentiation.Conclusion Fermented ginseng extract promotes zebrafish pancreatic β-cell regeneration. This regenerative action primarily contributed by the differentiation of endocrine precursor cell.
