知识与财富的链接
为探究黑水虻幼虫粉(HILM)替代饲料蛋白对四川白鹅生长性能、抗氧化能力、抗体水平和血清生化指标的影响,选取64只健康状况良好的1日龄四川白鹅,随机分成4组,每组4个重复,每个重复4只,即对照组、2% HILM组、4% HILM组、8% HILM组,分别饲喂含0、2%、4%、8%黑水虻幼虫粉的等能等氮饲粮,试验期40 d,期间自由采食和饮水。结果表明：与对照组相比,HILM替代饲料蛋白可提高试验鹅只平均日增质量(ADG),其中2% HILM组效果明显(p＜0.05);各HILM替代组血清总抗氧化能力(T-AOC)和谷胱甘肽过氧化物酶(GSH-Px)呈上升趋势,其中8% HILM组血清GSH-Px上升明显(p＜0.05);二次免疫后1周,各HILM替代组可提高血清H5-R13和H7-R4的抗体滴度,其中2% HILM组血清H5-R13抗体滴度显著升高(p＜0.05),4% HILM组血清H7-R4抗体滴度显著升高(p＜0.05);各HILM替代组血清生化指标和器官指数变化无统计学意义(p＞0.05)。综合表明：黑水虻幼虫粉替代饲料蛋白能提高四川白鹅的生长性能和抗氧化能力,对禽流感疫苗免疫效果有增强作用,以2% HILM组为适宜替代量。
This study was conducted to investigate the effects of replacing feed protein by Hermetia illucens larvae meal (HILM) on growth performance, antioxidant capacity, antibody level and serum biochemical indexes of Sichuan white geese. A total of 64 healthy 1-day-old Sichuan white geese were randomly allocated into 4 groups with 4 replicates in each group and 4 geese in each replicate, namely Control, 2% HILM, 4% HILM and 8% HILM groups, and were fed with diets containing 0, 2%, 4% and 8% of HILM, respectively. The geese can access to food and water ad libitum in the 40 d of experiment period. The results showed that compared with the control group, the replacement of feed protein with HILM could increase the Average Daily Gain (ADG) of geese, with a significant difference in 2% HILM group (p < 0.05). The serum Total Antioxidant Capacity (T-AOC) and Glutathione Peroxidase (GSH-Px) in each HILM replacement group showed an increasing trend, and the serum GSH-Px in the 8% HILM group increased significantly (p < 0.05).
7 d after the 2nd immunization, the serum H5-R13 and H7-R4 antibody titer were increased in each HILM replacement group. The serum H5-R13 antibody titer in the 2% HILM group and the serum H7-R4 antibody titer in the 4% HILM group were both significantly increased (p < 0.05). There was no significant effect on serum biochemical indexes and organ indexes in each HILM replacement group (p > 0.05). In conclusion, HILM can improve the growth performance, antioxidant capacity and enhance the immunity effect of avian influenza vaccine of Sichuan white geese, and the suitable replacement level is 2%.
