知识与财富的链接
以探究前处理垃圾渗滤液作为去除高浓度硝态氮外加碳源的可行性为目的,建立SBR系统R0、R1(分别以无水乙酸钠、前处理垃圾渗滤液+无水乙酸钠作为碳源),采用模拟高浓度硝态氮废水培养获得快速高效反硝化活性污泥,考察了其脱氮效能并进行了分子生物学分析。结果表明：在PLL添加体积分数为10%时,R1系统在2.5 h内可将硝态氮几乎完全去除,反硝化速率高达58.05 mg·(g·h)−1,是R0系统的1.79倍;16S rDNA扩增子测序结果显示,R0、R1反应器内微生物种群类别较为相似,丰度位于前3位的优势反硝化菌分别为假单胞菌属(Pseudomonas)、陶厄氏菌属(Thauera)和Pannonibacter,但相对丰度存在差异;经qPCR测定,实验组R1中反硝化基因narG、nirK、nirS和norB的相对表达量显著高于对照组R0。前处理垃圾渗滤液作为外加碳源可以提高污泥反硝化活性。
In order to explore the feasibility of pretreated landfill leachate (PLL) as an additional carbon source for the removal of high concentration nitrate nitrogen, SBR system R0 and R1 were established(R0: anhydrous sodium acetate carbon source; R1: PLL + anhydrous sodium acetate carbon source). Efficient denitrifying activated sludge was obtained through cultivation with the simulated high concentration nitrate nitrogen wastewater and PLL addition from anaerobic biofilter. Then its denitrification efficiency was investigated, and the molecular biological was also analyzed. The results showed that nitrate nitrogen could be almost completely removed within 2.5 hours in R1 system at PLL addition volume fraction of 10%, and the denitrification rate reached 58.
05 mg·(g·h)−1, which was 1.79 times of R0. 16S rDNA sequencing showed that the microbial population structure of R0 and R1 reactors were comparatively similar. The dominant denitrifying bacteria in the first three abundances were pseudomonas, thauera and pannonibacter, but their relative abundances were different; The results of qPCR showed that the relative expression of denitrification genes narG, nirK, nirS and norB in experimental group R1 was significantly higher than that in control group R0. PLL as an additional carbon source could promote the sludge denitrification activity.
