Pyrolysis to biochar is an efficient technique of disposing organic solid wastes. However, pyrolysis treatment of the cattle manure (CM) containing high concentrations of Cu and Zn is scarcely investigated, and the environmental behaviors of Cu and Zn in the cattle manure biochar (CMB) accompanying with their ecological risk keep unknown. In this study, the occurrence speciation of Cu and Zn in CMB, their leaching characteristics and the ecological risk of CMB from different pyrolysis temperatures (350, 550 and 750^oC) were evaluated using spectrum technology (i.e., BET, TEM-SEAD, XPS, and FTIR), the leaching experiments, and the risk tests. Results show that carbonization of CM to CMB with an increasing temperature from 350 to 750^oC improves the pore structure of CM and enlarges the specific surface area from 1.15m²/g in raw CM to 5.51~195.90m²/g in CMB. The pH value of the CMB increases from 8.18 (350℃) to pH 10.14 (750℃). Importantly, the Cu concentration increases from 1.22mg/g in raw CM to 18.29~35.11mg/g in CMB while the Zn concentration elevates from 1.23mg/g to 18.58~31.24mg/g. Meanwhile, most of the Cu and Zn are oxidized to paramelaconite (Cu₄O₃) and zincite (ZnO), which significantly reduces the concentrations of Cu and Zn in forms of extractable ones with deionized water, DTPA-TEA-CaCl₂ and HNO₃-H₂SO₄ as extracting agents, respectively. Furthermore, many functional groups (i.e., phenolic hydroxyl, alkane, carboxyl, amide, etc.) can immobilize labile Cu through adsorption and complexation, but a high pyrolysis temperature (>550^oC) tends to arise a significant decrease in species and amounts of functional groups and promote the complete conversion of Cu ions and the formation of stable metal oxide bonds between inorganics and Cu or Zn ions. In summary, the pyrolysis temperature over 550^oC could dramatically reduce the leaching rates of Cu and Zn and mitigate the ecotoxicity of CMB, which can be a potential approach to dispose the CM wastes with high concentrations of Cu and Zn.