知识与财富的链接
生物体中大部分酶催化反应都需要辅因子参与,辅因子平衡对维持正常的细胞代谢至关重要,而辅因子失衡则会导致细胞生长和生产的紊乱。在微生物细胞工厂的构建中,通过调节辅因子代谢平衡来提高产物合成途径的效率,从而调控细胞生长与产物生产,使代谢流能够最大限度地流向目标产物,已经成为代谢调控的重要手段。目前常见的用于代谢调控的辅因子有NAD(P)H/NAD(P)+、辅酶、ATP/ADP等。围绕这几种辅因子的代谢途径及功能分类进行了综述,并总结了微生物中不同产物利用辅因子平衡策略进行合成调控的研究,以期为各类化合物的高效生物合成提供参考。
Most of the enzyme-catalyzed reactions in organisms require the participation of cofactors. The balance of cofactors is essential to maintain normal cellular metabolism, and the imbalance of cofactors can lead to the inhibition of cell growth and production. In the construction of microbial cell factories, it has been considered to be an important strategy to regulate the balance of cofactor metabolism since the cofactor regulation could be employed to improve the efficiency of synthetic pathway of products and thus balance the cell growth and products synthesis, achieving the maximum metabolic flux towards the target products. Common cofactors currently used for metabolic regulation include NAD(P)H/NAD(P)+, coenzyme, and ATP/ADP. The metabolic pathways and functional classifications of these cofactors are reviewed, and the studies on the synthesis regulation of different products in microorganisms using cofactor balancing strategies are summarized. This paper will provide references for the efficient biosynthesis of various compounds.
