The macroporous resin was used to preliminarily purify the sterols of Korean pine seeds, and then the magnetic field assisted solvent crystallization was used to purify it for the second time. By comparing the adsorption and desorption effects of five macroporous resins on Korean pine seed oil sterol, AB-8 resin was selected as the best purification medium. The optimal process conditions for purifying Korean pine seed oil sterol were as follows: loading mass concentration of 0.2 mg/mL, loading volume of 30 mL, adsorption flow rate of 2 mL/min, adsorption time of 3 h, ethanol volume fraction of desorption solution 80%, ethanol volume of 70 mL, desorption flow rate of 2 mL/min, the desorption time was 4 h, and the purity of sterol in Korean pine seed oil was (45.32±2.13) % under these conditions. The optimum technological parameters for purification of Korean pine seed oil sterol by magnetic field assisted solvent crystallization were as follows: magnetic field intensity 0.3T, anhydrous ethanol as crystallization solvent, solid-liquid ratio 1∶10 g/mL, crystallization temperature 5 ℃, crystallization time 4 h. under these technological conditions, the purity of Korean pine seed oil sterol was (83.12 ± 3.24) %. After spectral scanning, the characteristic peak of purified sterol appeared at 210 nm; it has the characteristic absorption peak of phytosterol after infrared spectrum scanning analysis. Two kinds of phytosterols ( β-sitosterol and campesterol ) were identified by gas chromatography-mass spectrometry, and β-sitosterol account for more than 80% of the total amount of Korean pine seed oil sterol.