ObjectiveTo construct a service quality evaluation system of physical examination centers in chain hospitals based on mysterious customer experience method, and provide a reference for the medical group to evaluate and improve the service quality of physical examination centers in its chain hospitals.MethodsThe service quality evaluation form and the first draft of the evaluation scheme of physical examination centers in chain hospitals based on the mysterious customer experience method were prepared by literature review. The Delphi method was used to invite 25 experts to conduct two rounds of expert letter consultation to construct the evaluation form. Furthermore, 10 experts were selected to conduct opinion consultation to establish the evaluation scheme and form the evaluation system.ResultsFinally, an evaluation table containing 8 key links of service quality and 59 service quality evaluation indicators was formed; a mysterious customer experience evaluation plan with 41 on-site and 11 experts' implementation standards was formed. Conclusion The service quality evaluation system of physical examination centers in chain hospitals based on the mysterious customer experience method constructed in this study is scientific, and the evaluation indicators and evaluation scheme are comprehensive, which can provide guidance for the supervision and improvement of service quality of physical examination centers in chain hospitals of medical groups.