知识与财富的链接
〔摘 要〕 目的：建立炉甘石氧化锌洗剂中苯酚的含量测定方法。 方法：采用高效液相色谱法（HPLC）,色谱柱为 Welch Xtimate? C18（5 μm,4.6 mm×150 mm）,以甲醇 – 水（40:60）为流动相进行等度洗脱,流速 1.0 mL·min-1,柱温 30 °C,进样量 10 μL,检测波长 270 nm。 结果：苯酚在 62.08 ~ 434.56 μg·mL-1 线性关系良好（r ＞ 0.999）,加样回收率 为 100.46 %,相对标准偏差（RSD）为 0.28 %。 结论：本研究建立的方法简便、快速、准确,重现性好、精密度高。可用 于炉甘石氧化锌洗剂中苯酚的质量控制。
〔Abstract〕 Objective To establish a method for the determination of phenol in calamine zinc oxide lotion. Methods High performance liquid chromatography (HPLC) was used, the chromatographic column was Welch Xtimate? C18 (5 μm, 4.6 mm×150 mm), methanol-water (40:60) was used as the mobile phase for isocratic elution, the flow rate was 1.0 mL·min-1, column temperature was 30 °C and injection volume was 10 μL. The detection wavelength was 270 nm. Results Phenol had a good linear relationship in the range of 62.08 ~ 434.56 μg·mL-1 (r > 0.999), the sample recovery rate was 100.46%, and the relative standard deviation (RSD) was 0.28%. Conclusion The method established in this study is simple, fast, accurate, reproducible, and highly precise. It can be used for the quality control of phenol in calamine zinc oxide lotion.
