知识与财富的链接
从NCBI公共数据库下载获得27904条鹰嘴豆表达序列标签EST,去除其中低质量的和冗余序列后得到全长为6198092bp的11224条无冗余EST.利用SSRIT(Simple sequence repeat identification tool)软件共在这些序列中发掘出982个SSR,它们分布于865条EST中,出现频率为8.69%,平均长度为15.58bp,平均距离为6.31kb.在鹰嘴豆EST-SSR中,二核苷酸重复是最主要的重复类型(66.90%),其次是三核苷酸重复类型(30.75%),出现最多的重复基元类型是TA/AT(24.95%).
27 904 ESTs of chickpea (Cicer arietinum L.) were downloaded from the database of NCBI and some redundant or low quality sequences were removed. Finally, 11 224 non-redundant ESTs with total length of 6 198 092 bp were obtained. SSRIT (Simple sequence repeat identification tool), a web tool for SSR hunting, was used to analyze the sequences. The results showed that a total of 982 SSRs distributed in 865 ESTs were detected, with the frequency for the non-redundant ESTs being 8.69%. The average length and distribution distance of the EST-SSRs were about 15.58 bp and 6.31 kb. Among the different SSRs ranging from 2 to 7 bp, dinucleotide repeats were most abundant (66.90%), followed by trinucleotide repeats (30.75%). Among 83 identified motif types, TA/AT had the highest EST frequency (24.95%). The results would contribute to the development of EST-SSR markers in chickpea.