3D bio-printing, a process of converting medical images acquired from clinical data into two-dimensional mode using digital imaging technology, is used for computer-aided design/computer-aided manufacturing software to print and overlay layer by layer. In this bottom-up printing process, biomaterials and living cells are precisely mapped to the target location to achieve sophisticated printing of complex structures. Based on the high flexibility of 3D printing, this high and new technology can be used to repair the problems caused by trauma, tumor or heredity. Therefore, 3D-bioprinting have become a research attraction in recent years. This paper summarized recent research progress of pure biological material in 3D bio-printing and cell-printing, including the characteristics of 3D-biological printing and the way of 3D-biological printing as well as the clinical maxillofacial application and its future development.