知识与财富的链接
多环芳烃（PAHs）危害人类健康与生态系统,其土壤修复备受关注.生物修复法因环保优势而广泛的应用,但修复效果受PAHs降解菌（PDB）较低的丰度与活性限制.本研究构建土壤微生物电解池（SMEC）以增强PDB活性,进而提升修复效果.实验结果表明,900-SMEC使菲（PHE）和芘（PYR）去除效果分别提升至42.6%±1.6%和30.9%±0.6%.同时,900-SMEC输出电流达1.4 mA,为500-SMEC的1.8倍.外加电压增加促进微生物活性,降低电荷与扩散内阻,利于电能输出和PAHs去除.微生物群落分析显示,SMEC显著提升关键PDB丰度,与PAHs降解相关基因的表达量也增加.因此,SMEC是一种提高PAHs原位生物修复效果的新方法.
Polycyclic aromatic hydrocarbons （PAHs） pose substantial threats to both human health and ecosystems, thereby rendering the remediation of PAHs-contaminated soils a considerable attention. Bioremediation methods have gained wide application due to their environmentally friendly characteristics, but the remediation efficiency is significantly hindered by the low abundance and activity of PAHs-degrading bacteria （PDB）. In this study, soil microbial electrolysis cells （SMEC） were constructed with the aim of enhancing the activity of PDBs and thus improving PAHs removal efficiency. The results showed that the 900-SMEC increased the removal efficiency of phenanthrene （PHE） and pyrene （PYR） to 42.6% ± 1.6% and 30.9% ± 0.6%, respectively. Meanwhile, the 900-SMEC generated the highest current of 1.4 mA, which was 1.8 times to that of the 500-SMEC. An increase in applied voltage was found to boost microbial activity and reduce charge and diffusion resistances within the system, thereby facilitating both power generation and PAHs removal efficiency. Microbial community analysis revealed that SMEC significantly increased the abundance of typical PDB. The copies of genes related to PAHs degradation were concurrently increased. In conclusion, SMEC represents a novel method to enhance the in-situ bioremediation efficiency of PAHs-contaminated soils.
