知识与财富的链接
目的探索NGLxcf-3000血细胞分离机针对高红细胞压积(hematocrit,HCT)的采集者采集富血小板血浆(platelet-rich plasma,PRP)时,通过提前补液稀释血液的处理方式,解决PRP产品冲红问题的可实施性、安全性以及产品质量的实用性,为PRP的安全采集和产品的质量控制提供一定参考。方法统计2024年1月—6月在血站采集PRP产品患者资料378例,其中358例正常范围HCT(0.36~0.50)作为对照组,20例高HCT(>0.52)作为试验组,试验组PRP采集前、采集中进行生理盐水补液稀释血液采集法。通过采集时间、循环血量、抗凝剂使用量、采集量以及不良反应发生的差异,PRP产品中血小板含量、红细胞和白细胞残存量等指标对比分析两组的差异。结果① 试验组平均采集时间为(24.83±9.94) min、循环血量(669.00±277.68) mL、抗凝剂用量(71.61±26.41) mL、采集量(43.61±6.34) mL, 与对照组比较,差异无统计学意义(P>0.05);②对照组358例采集者中有2例出现心慌反应,试验组20例采集者未出现不良反应,两组差异无统计学意义(P>0.05);③对照组产品血小板计数、白细胞、红细胞残余量分别为(1 136.28±271.70)×109/L,(0.79±0.58)×109/L、(0.02±0.01)×1012/L;试验组产品血小板计数、白细胞、红细胞残余量分别为(1 022.00±373.58)×109/L、(0.93±0.42)×109/L、(0.02±0.01)×1012/L,两组差异无统计学意义(P>0.05)。结论静脉补液稀释血液法用于高HCT患者,可解决PRP采集中产品冲红问题,此法简易方便,安全可行。
ObjectiveTo evaluate the feasibility, safety, and quality control practices associated with platelet-rich plasma(PRP) product flushing by employing the NGLxcf-3000 blood cell separator to pre-dilute blood from hematocrit(HCT) collectors. The findings provide valuable insights into safe PRP collection and product quality management.MethodsData were collected from 378 patients who received PRP products at our blood bank between January and June 2019; among these, 358 patients with hematocrit levels within normal range (0.36-0.50) constituted the control group, while 20 patients with HCT>0.52 formed the experimental group. Prior to and during PRP collection, blood in the experimental group was diluted using a normal saline replenishment method. We compared and analyzed differences in collection time, circulating blood volume, anticoagulant dosage, collection volume, incidence of adverse reactions, as well as platelet content alongside residual red and white blood cells in PRP products between both groups.Results① No significant differences were observed regarding average collection time ([24.83±9.94]min), circulating blood volume ([669.00±277.68] mL), anticoagulant dosage ([71.61±26.41] mL), or total collected volume ([43.61±6.34]mL) when comparing the experimental group to controls(P>0.05); ②Amongst the control group's 358 participants, two experienced palpitations; conversely, no adverse reactions occurred within the experimental cohort-indicating no significant difference between groups(P>0.05); ③The control group's platelet content measured at an average of (1 136.28±271.70)×109/L with white cell residues at approximately (0.79±0.58)×109/L and red cell residues at about (0.02±0.01)×1012/L; whereas test groups exhibited averages of platelet content at (1 022.00±373.58)×109/L with white cell residues at approximately (0.93±0.42)×109/L and red cell residues at about (0.02±0.01)×1012/L, and there was no statistically significant difference between the two groups(P>0.05).ConclusionThe method of intravenous fluid infusion for blood dilution is applied to patients with high hematocrit of red blood cells, which can solve the problem of product redness during the collection of platelet-rich plasma(PRP). This method is simple and convenient, and it is safe and feasible.
