The Jiaojia gold deposit is a typical representative altered rock type gold deposit in the Jiaodong Peninsula, with a gold reserve of more than 200 tons. The distribution of the vein-like orebodies is controlled by the Jiaojia Fault. The potash feldspathization, beresitization, silicification and carbonization are well developed in the country rock. Pyrite is the main gold-bearing mineral in the ore. The hydrothermal metallogenic period can be divided into four stages:(i) pyrite-quartz stage; (ii) gold-quartz-pyrite stage; (iii) gold-quartz-polymetallic sulfide stage; and (iv) quartz-carbonate stage. In this paper, we collected nine pieces of drilling hole samples at different depths (-600~-1100m) from the No. I orebody of Jiaojia deeply-seated gold deposit. The trace elements and S-He-Ar isotopic compositions of the pyrites in these samples are analyzed, and the compositions, properties and sources of their ore-forming fluids are discussed. It is found that the pyrites of the No. I orebody are light yellow, with strong metallic luster, euhedral-granular or hypidiomorphic-granular textures, and they ocuured as disseminated grains with sizes of 50~1000μm or veined assemblages in sericitized cataclastic rocks, which are taken as minerals of the gold-quartz-pyrite metallogenic stage (ii). The contents of their REE are relatively low and enriched in LREE, with ΣREE of 3.27×10^-6~38.5×10^-6, δCe values of 0.81~1.05, δEu values of 0.67~1.43 and the ratios of Th/La, Nb/La less than 1, indicating the ore-forming fluids might be Cl-rich reduced fluids. The Co/Ni ratios of the pyrites range from 0.54 to 1.57, with an average value of 0.99, these ratios are close to average values of those from metamorphic-hydrothermal type gold deposit. The Y/Ho ratios range from 21.9 to 35.5, coincident with the mantle (25~30) and the continental crust of eastern China (20~35), indicating that the ore-forming fluids were related to the crust-mantle interaction. The Y/Ho, Zr/HF and Nb/Ta values vary in a wide range, indicating that the metasomatism or the addition of external hydrothermal solution. Compared with the continental crust, pyrite is rich in chalcophile elements and siderophile elements such as Cu, Pb, Zn, Cd, Co, Ni, and depleted of high field strength elements (HFSE). The δ³⁴S values of the pyrites in the Jiaojia deeply-seated gold deposit vary within a range between 7.5 ‰ and 9.8 ‰, with an average value of 8.7 ‰, which is lower than that of shallow deposit, showing the characteristics of decreasing δ³⁴S value from shallow to deep. The sulfur isotopic compositions of the Jiaodong gold deposits are similar as a whole, the δ³⁴S values of most deposits are positive, and vary mainly in the range of 6‰~12‰. The source of δ³⁴S is related to the ancient metamorphic basement rocks, Mesozoic granites and mantle-derived fluids. The published data showed that the variations of the δ³⁴S values in this area are in this order:quartz vein type < sulfide quartz vein type < altered rock type, which means that the difference of metallogenic type is the most important factor causing the different sulfur isotopic compositions. The ³He/⁴He values of the pyrites are 1.6±0.1Ra~1.8±0.1Ra, and their ⁴⁰Ar/³⁶Ar values are 750~3106, showing the characteristics of a mixed source. The proportion of mantle-derived helium is 20%~22%, which indicate that mantle-derived fluids are involved in the metallogenic process. The mineralization of the Jiaojia gold deposit is closely related to the strong crust-mantle interaction in eastern China in the Late Yanshanian Period.