知识与财富的链接
从北京通州地区芹菜软腐病样中分离获得的菌株QC01在实验室条件下能浸软芹菜叶柄组织,并能引起温室种植的芹菜软腐症状的产生。经病原菌表型特征分析,确定该菌株为有鞭毛的革兰氏阴性果胶杆菌Pectobacterium。QC01与参试的P.carotovorum subsp.odoriferum(Pco)菌株T5和CC1均具备在37℃以及在含有7%Na Cl培养基中生长的能力;能分解柠檬酸盐和液化明胶;除常见碳源外,QC01还能降解α-甲基葡糖苷、麦芽糖、山梨醇、阿拉伯糖和阿拉伯半乳聚糖。基于16S rRNA基因完整序列系统发育关系表明,QC01与其他Pco菌株聚集成明显的Pco类群。QC01和CC1 type II与已报道的Pco菌株JKI 582 type II和NB 1892的16S rRNA基因完整序列相似性为100%。这是首次在中国发现Pectobacterium carotovorum subsp.odoriferum引发芹菜软腐病。
The strain QC01 isolated from soft rotted samples of celery in Beijing Tongzhou District, it could macerate tissues of celery petioles in vitro inoculated under the laboratory conditions, and cause soft rot symptoms on celery in vivo planted in the greenhouse. Morphological analyses showed that QC01 was a Gram-negative, rod-shaped Pectobacterium with flagellum. QC01 together with P. carotovoum subsp. odoriferum （Pco）strains CC1 and T5 could grow at 37℃ and even in 7% NaCl contained medium; could utilize 〖WTBZ〗α〖WTB1〗-methyl glucoside, maltose, sorbitol, arabitol and arabinogalactan and other regular carbon sources. In the phylogenetic analysis of the whole 16S rRNA gene sequences, QC01 and other Pco strains grouped together as a distinct Pco cluster. The entire 16S rRNA gene sequence of QC01 had a similarity of 100% to those of two reported Pco strains JKI 582 type II and NB 1892. It was the first report that Pectobacterium carotovorum subsp. odoriferum caused soft rot disease on celery in China.
