In order to make clear the immune effect of feline herpesvirus type 1（FHV-1）vaccines and the status of infection with FHV-1 in stray cats,a detection method for neutralizing antibody against FHV-1 was established by the clinical isolates,and 20 serum samples from clinical pet cats with vaccination against FHV-1 and 10 serum samples from stray cats without vaccination in zoos were detected for neutralizing antibody. The results showed that the qualified rates of antibodies were both 60% in the two kinds of cats,which indicated that the immune effect was poor,and FHV-1 infection of stray cats without vaccination was serious. Therefore,surveillance for the antibodies against FHV-1 should be strengthened with timely supplement of vaccination,meanwhile,stray cats should be controlled,sheltered,vaccinated and monitored. Some references were consequently provided for prevention and control of FHV-1.