知识与财富的链接
目的 建立高效液相色谱(HPLC)法同时测定复方阿胶补血颗粒中4种氨基酸。方法 采用Waters Sunfire C18色谱柱（4.6 mm × 250 mm,5 mm）,以乙腈-0.1 mol·L-1醋酸钠溶液（用36%乙酸调节pH值至6.5）（7∶93）为流动相A,以乙腈-水（4∶1）为流动相B,梯度洗脱（0~13 min,100% A ® 93% A;13~17.9 min,93% A ® 88% A;17.9~29 min,88% A ® 85% A;29~39 min,85% A ® 66% A;39~45 min,66% A ® 0% A）,柱温43 °C,体积流量1.0 mL·min-1,检测波长254 nm。结果 L-羟脯氨酸、甘氨酸、丙氨酸、L-脯氨酸进样量分别在0.012 ~ 0.117、0.022 ~ 0.218、0.010 ~ 0.097、0.016 ~ 0.160 mg范围内与色谱峰峰面积呈良好的线性关系;平均回收率（n=6）分别为96.4%、97.3%、97.1%、99.4%;RSD分别为1.2%、1.9%、1.7%、0.9%。结论 该方法操作简单,重复性好,为控制复方阿胶补血颗粒的质量提供了可靠的方法。
Objective To establish an HPLC method for simultaneous determination of 4 amino acids in Fufang Ejiao Buxue Granules.Methods The quantitative analysis was carried out with a Waters Sunfire C18 column (4.6 mm×250 mm,5 mm).A binary mobile solvent was used: mobile solvent A was acetonitrile – 0.1 mol·L-1 sodium acetate solution (Adjusting pH to 6.5 with 36% acetic acid) (7:93) and mobile solvent B was acetonitrile – H2O (4:1). The mobile phase was delivered at a flow rate of 1.0 mL·min-1 with a gradient elution profile (0~13 min, 100%A ® 93%A; 13~17.9 min, 93%A ® 88%A; 17.9~29 min, 88%A ® 85%A; 29~39 min, 85%A ® 66%A; 39~45 min, 66%A ® 0%A). The column temperature was at 43 ℃.The detection wavelength was 254 nm.Results The linear ranges of L-hydroxyproline,glycine,alanine,and L-proline fell within the ranges of 0.012~0.117,0.022~0.218,0.010~0.097,0.016~0.160 mg,respectively. The average recoveries (n=6) were 96.4%,97.3%,97.1%,and 99.4%;The relative standard deviations were 1.2%,1.9%,1.7%,and 0.9%,respectively.Conclusion The method was simple and reproducible,which could be used to the quality control of Fufang Ejiao Buxue Granules.
