知识与财富的链接
从富硒蛹虫草中提取硒多糖和硒蛋白。分别采用ICP-MS 法和苯酚硫酸法及考马斯亮蓝法对硒多糖和硒蛋白中硒、多糖及蛋白含量进行测定;在Fenton 体系中,以510nm 波长处的吸光度研究硒多糖和硒蛋白对羟自由基的清除作用。结果表明：硒多糖和硒蛋白清除羟自由基的效果强于相同浓度的无机硒、多糖及蛋白;其中,富硒蛹虫草硒多糖的对羟自由基最高清除率为38.02%,而硒蛋白的最高清除率可达75.00%。
In this study, selenium polysaccharide and selenium protein were extracted from selenium-rich Cordyceps militaris by modern separation techniques. The contents of selenium, polysaccharide and protein in Se-polysaccharide and Se-protein were determined by ICP-MS, phenol-sulfuric acid method and Coomassie brilliant blue method. In addition, the scavenging capabilities of Se-polysaccharide and Se-protein on hydroxyl free radicals were evaluated by the absorbance at 510 nm in Fenton system. Results indicated that the scavenging activities of Se-polysaccharide and Se-protein were obvious higher than those of inorganic selenium, polysaccharide and protein under the same condition. The highest scavenging rates of Se-polysaccharide and Seprotein were 38.02% and 75.00%, respectively.
