知识与财富的链接
将新鲜山柰、海南三七块根的切片置于DXR 激光共焦显微拉曼光谱仪下,用10倍物镜观察到山柰、海南三七的油细胞。将激光聚焦在油细胞上,获得了山柰、海南三七油细胞中的精油拉曼光谱,不同油细胞上获得的拉曼光谱非常相似。山柰的拉曼光谱与甲氧基肉桂酸乙酯的拉曼光谱,海南三七的拉曼光谱与苯甲酸苄脂的拉曼光谱在峰形、峰位上都非常相似。采用密度泛函理论（DFT）计算了甲氧基肉桂酸乙酯及苯甲酸苄脂拉曼光谱,计算的拉曼光谱与实验的拉曼光谱非常相似,据此对甲氧基肉桂酸乙酯拉曼光谱和山柰油细胞中的精油拉曼光谱及苯甲酸苄脂拉曼光谱和海南三七油细胞中的精油拉曼光谱进行了初步的归属,说明山柰的主要挥发物为甲氧基肉桂酸乙酯,海南三七的主要挥发物为苯甲酸苄脂。
To identify the ingredient in the oil cells of Kaempferia galanga L. and Kaempferia rotunda L. at room temperature, and to avoid sample pretreatment and extractions which can be labor intensive, the work showed the possibility to detect in situ the main components of the essential oils in the two plants oil cells by Raman spectroscopy. Under the DXR laser confocal micro Raman spectrometer, the oil cells could be seen with objective lens of 10×. Different oil cells had similar Raman spectrum. The Raman spectrum of 4-methoxycinnamic acid ethyl ester and benzyl benzoate was obtained. The Raman spectrum of K. galanga L. was correlated very well with that of 4-methoxycinnamic acid ethyl ester, and that of K. rotunda L. was correlated very well with that of benzyl benzoate. The Raman spectroscopy of 4-methoxycinnamic acid ethyl ester and benzyl benzoate was calculated by the density functional theory (DFT). The Raman spectroscopic bands were assignment with the help of calculation. It indicated that the essential principal oil components of K. galanga L. and K. rotunda L. in the oil cells were 4-methoxycinnamic acid ethyl ester and benzyl benzoate.
