A silver-bearing copper ore in Yunnan is with copper grade of 0.78%, silver grade of 70.27 g/t and sulfur grade of 19.83%. Flotation tests were carried out based on the process mineralogy research in order to develop and utilize the resources efficiently. The results showed that: ①Copper in the ore mainly exists in the form of chalcopyrite, and the disseminated grain size varies greatly with medium and coarse grains as the main grain size, ranging from 0.01 mm to 1 mm. More than 80% of the ores particles distribute in the grain size ranging from 0.019 mm to 0.45 mm. Tetrahedrite shows fine-disseminated, with more than 85% of which distribute in the -0.037 mm grain size. Silver mainly occurs in tetrahedrite and sulfur mainly exists in the form of pyrite. ②When the grinding fineness of the ore is -0.074 mm accounting for 80.3%, copper and sulfur mixed concentrate was obtained by the two roughing-one scavenging process. When the grinding fineness of the copper and sulfur mixed concentrate is -0.037 mm accounting for 73.8%, copper and sulfur were separated by the one roughing-two cleaning-one scavenging process. Copper concentrate with copper grade of 22.37%, copper recovery rate of 90.28%, silver content of 1 221.51 g/t and silver recovery rate of 54.72% was obtained. Sulfur concentrate with sulfur grade of 41.86%, sulfur recovery rate of 90.12%, silver content of 55.75 g/t and silver recovery rate of 33.87% was obtained. The separation and enrichment of copper, sulfur and silver were well realized.