知识与财富的链接
目的评价丹麦ROSCO酵母菌纸片扩散法药敏试验及其判定标准在我院应用的可行性。方法以美国临床实验室标准化委员会（NCCLS）酵母菌纸片扩散法药敏试验为参照，采用丹麦ROSCO纸片扩散法药敏试验检测氟康唑对80株临床分离酵母菌的药敏试验。结果ROSCO纸片扩散法药敏试验选取基本判读标准，氟康唑药敏结果与NCCLS结果差异无统计学意义（P〉0．05）；而ROSCO选取严格判读标准，白念珠菌的氟康唑药敏结果与NCCLS结果存在差异，热带念珠菌结果差异无统计学意义（P〉0．05）。结论丹麦ROSCO酵母菌纸片扩散法药敏试验基本判读标准结果与美国NCCLS判读结果相符合，适合我院临床菌株的结果判读；而对于严重系统感染菌株所选取的严格判读标准，不适合白念珠菌的结果判读。
Objective To evaluate antifungal susceptibility test and criteria by Denmark Rosco disk diffusion method.Methods With reference of the NCCLS disk diffusion method, susceptibility of 80 clinical yeast isolates to Fluconazole was tested by Denmark Rosco disk diffusion method and the NCCLS disk diffusion method.Results Rosco disk diffusion choosing according to basic criteria, the results of Rosco Disk Diffusion have no statistical significance compared with the results of NCCLS Disk diffusion (P>0.05). But Rosco disk diffusion choosing by the more stringent criteria of Rosco, have possessed significance for Candida albicans , not for Candida tropicalis compared with the results of NCCLS Disk Diffusion(P>0.05).Conclusion The basiccriteria of Rosco Disk Diffusion is suitable for result evaluation of clinical yeast isolates in our hospital. And the stringent criteria is not suitable for result evaluation of Candida albicans.
