知识与财富的链接
从大肠杆菌感染的家蚕蛹提取RNA,用RT-PCR方法扩增未知抗菌肽基因片段,经过克隆测序,获得了蚕抗菌肽A基因的部分片段164 bp,为制备蚕抗菌肽A基因探针,筛选基因文库打下了基础.
Total RNA was prepared from fat body of the silkworm, Bombyx mori, 9 hours after injected by E.coli K12D31. Single-strand cDNA was synthesized by reverse transcription (RT). Partial fragments of cecropin A cDNA were obtained, cloned and sequenced, by PCR technique with a pair of degenerate primers designed according to the amino acid sequence of CM4 in Bombyx mori and cecropin A in Hyalophora. These laid a foundation for further research on preparation of the probes of cecropin A for screening the silkworm cDNA library.