知识与财富的链接
目的:研究TgN(p53mt-LMP1)/HT转基因小鼠鼻腔和鼻咽黏膜上皮细胞增殖与p16、c-jun基因表达的关系.方法:TgN(p53mt-LMPI)/HT小鼠和野生型C57BL/6J小鼠分别分为诱癌组和对照组,即TgN(p53mt-LMP1)/HT诱癌组(TI)、TgN(p53mt-LMP1)/HT对照组(TC)、C57BL/6J诱癌组(CI)和C57BL/06J对照组(CC)4组,H-E染色法观察各组小鼠鼻腔和鼻咽黏膜上皮细胞增殖特征,比较癌前病变率;免疫组织化学染色法检测各组小鼠鼻腔和鼻咽黏膜上皮组织中p16、c-jun基因的表达水平.结果:TI组小鼠鼻腔或鼻咽黏膜上皮细胞非典型性增生明显,TI、TC、CI和CC组小鼠鼻腔和/或鼻咽黏膜上皮癌前病变卒分别为90%、10%、0和0.与TC、CI和CC组相比,TI组鼻腔和鼻咽上皮细胞p16基因表达水平显著降低(P＜0.01)和c-jun基因表达水平显著增高(P8＜0.01);与CC组相比,TC组两基因的表达也具有同样的变化趋势(P＜0.01).结论:TgN(p53mt-LMPI)/HT小鼠鼻腔和鼻咽黏膜上皮细胞增殖活性增加,二亚硝基哌嗪可提高TgN(p53mt-LMP1)/HT小鼠鼻腔和鼻咽黏膜上皮细胞的增殖活性,而细胞增殖活性增加与p16基因表达水平下调和c-jun基因表达水平上调密切相关.
Objective: To investigate the relationship between the proliferation of nasal and nasopharyngeal epithelium with the expression of p16 and c-jun genes in TgN (p53mt-LMP1)/HT transgenic mice. Methods: TgN (p53mt-LMP1)/HT transgenic mice and the same strain of C57BL/6J wild-type mice of 5 months old were randomly divided into two groups: cancer group and the control group. The animals were also divided into 4 groups in total: the TgN (p53mt-LMP1)/HT cancerous lesion-inducing group (TI), the TgN (p53mt-LMP1)/H...
