知识与财富的链接
[目的]测定不同来源胡黄连主根与须根中香草酸和肉桂酸含量.[方法]甲醇-0.1%磷酸(40:60)为流动相,TIANHE-C1S(250 nm×4.6 nm,10μm)为色谱柱,检测波长260 nm,流速:1 mL/min,柱温25℃.[结果]香草酸、肉桂酸线性范围分别为0.72～72 la,g(r=0.999 8)、0.35～3.50μg(r=0.9999),平均回收率分别为99.62%[平均标准偏差(RSD=0.75%)1、98.84%(RSD=1.65%).胡黄连的主根与须根均含有香草酸与肉桂酸.[结论]该法快速、简便、精密度、重复性、稳定性好,适用于胡黄连含量分析测定.
[Objective] To establish HPLC method for the determination of Vonillic acid and cinnamic acid in rhizoma and fibre of picro-hiza Scrophulariiflora Pennell.[Methods] The column was TianHe C18 (150 mm×4.6 mm) with 10 μm packing. The mobile phase used was methanol-phosphorus acid(40:60). The flow rate was 1.0mL·min-1, detection Wavelength was 260 nm. [Results] The linearity of this method was good in the ranges of 0.72-72 μg for Vonillic acid (r=0.999 8);0.35-3.50 μg for cinnamic acid (r=0.9999). The recoveries of Vonillic acid and cinnamic acid were 99.62% and 98.84%. RSD were 0.75% and 1.65% respectively. [Conclusion] The method is accu-rate, precise and suitable to determine Vonillic acid and cinnamic acid in Picrohiza Scrophulariiflora Pennell.
