Four kinds of selective media MRS, MRS-sorbitol, MRS-5.2 and Elliker were used, adopting spreading plate method and pouring inoculation method, under the conditions of candle-desiccator method (oxygen deficiency), seal-parafilm method (micro-oxygen), and oxygen supplying method to study the selective counting methods of lactic acid bacteria (Lactobacillus burglarious, Streptococcus thermophile, Lactobacillus casei, and Bifidobacterium) in four kinds of market-selling yogurt fermented with multi-strain. The results showed that candle-desiccator method could fairly reflect the practical conditions of live bacterial quantity of lactic acid bacteria followed by seal-parafilm method; and under different cultivation conditions four kinds of selective media were sensitive for counting live lactic acid bacteria; while spreading plate method and pouring method to count live bacteria had significant differences; and pouring method was superior to spreading plate method.