As an important resistant gene in tomato, Mi-1  gene shows potential for resistance to Meloidogyne spp., Macrosiphumeuphorbiae and Bemisia tabaci. The principle of such resistance should be attributed to an encoded protein, which is of CC-NBS-LRR structure, being of broad spectrum for resistance towards plant diseases and pests. In this paper, we reviewed its characteristics of insect-resistance; discussed the gene structure and the insect-resistant types of its products; analyzed the genes resistant ability towards Meloidogyne spp., Macrosiphum euphorbiae and Bemisia tabaci; and further studied the resistant mechanisms of Mi-1  by means of gene mutation and salicylic acid pathway. Through objective evaluation on the functional mechanism of plant endogenous Mi-1  gene for plant diseases and pests, this study definitudes the application value of Mi-1  in agricultural production, thus provides scientific reliance for biological resistance control towards plant diseases and pests.