知识与财富的链接
利用3’和5’RACE技术从中国对虾Fenneropenaeus chinensis肝胰腺中克隆了1个血蓝蛋白基因FCHc,FCHc基因cDNA全长为2161bp。其中,开放阅读2 034bp,编码678个氨基酸,预测分子量为77.59 kDa。FCHc序列与凡纳滨对虾Litopenaeus vannamei血蓝蛋白同源性为82%,与日本囊虾Marsupenaeus japonicas同源性为85%。Real-timePCR实验结果表明,FCHc在肝胰腺中的相对表达量最高,在心脏和表皮中几乎不表达。该基因在鳗弧菌和对虾白斑综合征病毒(WSSV)感染后的对虾肝胰腺中的表达量显著增加,并具有不同的时空表达趋势,提示中国对虾FCHc基因在免疫反应中具有重要作用。
A hemocyanin gene(FCHc) was cloned from hepatopancreas of Chinese shrimp Fenneropenaeus chinensis by Rapid Amplification Complementary DNA Ends(RACE) method.The full length cDNA of FCHc consists of 2 161bp with a 2 034 bp Open Reading Frame(ORF),which encoded 678 amino acids,and the predicted molecular mass was 77.59kDa.It showed 82% and 85% homology with those of Litopenaeus vannamei and Marsupenaeus japonicas.The real-time PCR results showed that the expression level of FCHc in hepatopancreas was the high...
