知识与财富的链接
目的 评估16S rRNA基因检测在自发性细菌性腹膜炎(SBP)快速诊断中的应用价值.方法 采用16S rRNA基因荧光定量多聚酶链反应检测76例疑似SBP及6例非感染性腹腔积液患者腹水细菌DNA,并与腹水细菌培养结果进行比较.结果 16S rRNA检测疑似SBP患者腹水标本的阳性率为22.4%,明显高于腹水细菌培养的7.9%(P<0.01) .6例非感染性腹腔积液患者的腹水16S rRNA基因荧光定量PCR检测结果和细菌培养结果均为阴性.结论 16S rRNA检测可做为SBP的快速诊断方法,其敏感性优于腹水细菌培养.
ObjectiveTo evaluate the value of ascitic bacterial 16S rRNA gene determination in the rapid diagnosis of spontaneous bacterial peritonitis (SBP). Methods16S rRNA gene from bacterial DNA in ascites was determined by quantitative fluorescent polymerase chain reaction (PCR) in 76 patients with suspected SBP and 6 patients with non-infectious ascites. The results were compared with those obtained from bacterial culture. ResultsThe positive rate of SBP was 22.4% among patients detected with ascitic bacterial 16S rRNA gene determination-based quantitative fluorescent PCR, which was significantly higher than that (7.9%) in patients only received bacterial culture (P < 0.05). In addition, in 6 patients with non-infectious ascites, both the 16S rRNA gene determination-based quantitative fluorescent PCR and bacterial culture showed negative results. Conclusions16S rRNA gene determination-based quantitative fluorescent PCR can be an effective tool for the rapid diagnosis of SBP. It is more sensitive than the bacterial culture.
