知识与财富的链接
目的探讨三氧化二砷(As2O3)对人胃癌细胞株MKN-28抑制生长及诱导凋亡的生物学效应。方法采用光镜观察、溴化二甲噻唑二苯四氮唑(MTT)还原法、流式细胞仪(FCM)检测法检测As2O3在不同作用时间、不同给药浓度时对MKN-28细胞生长的影响。结果MKN-28细胞经As2O3作用后,细胞增殖受到明显抑制,而且呈浓度-作用时间依赖关系。流式细胞仪检测结果显示DNA直方图上出现典型的亚二倍体凋亡峰,细胞周期分析发现药物作用后MKN-28细胞的生长周期受到明显阻滞。结论As2O3既能有效地抑制人胃癌细胞株MKN-28的生长,又能诱导该细胞凋亡,而且呈浓度依赖性和作用时间依赖性。
Objective To study the biological effects of arsenic trioxide(As_2O_3) on human gastric cancer cells MKN-28 in vitro.Methods By means of light microscope,MTT reduction assay and flow cytometry to observe apoptosis of human gastric cancer cells in different effect time and concentration of As_2O_3.Results As_2O_3 could significantly inhibit the proliferation of MKN-28 cells and the inhibitory effect was dose-and-timed ependent.The cells treated by As_2O_3 showed a typical apoptotic morphology and hypodiploid peak before G_1 phase.Conclusion As_2O_3 not only could inhibit significantly the proliferation of MKN-28 cells but also could induce apoptosis of MKN-28 cells.
