知识与财富的链接
目的 评估白介素-11(Interleukin-11,IL-11)对小鼠异基因骨髓移植(allo-BMT) 后急性移植物抗宿主病(aGVHD) 的预防作用. 方法 用主要组织相容性抗原(MHC) 完全不合的纯种近交系SPF级小鼠,雄C57BL/6(H-2b)为供鼠,雌性BALB/C(H-2d)为受鼠,建立allo-BMT/aGVHD 动物模型,随机分3组:模型对照组、IL-11组(剂量为500μg/(kg·d)、环孢菌素A(CsA)组(剂量为10mg/(kg·d),观察其对aGVHD 的预防作用. 结果 IL-11组、CsA组受鼠生存时间均长于模型对照组(P<0.01);IL-11组、CsA组受鼠aGVHD临床表现和病理改变均较模型对照组明显轻.IL-11组、CsA组之间平均生存时间比较无统计学差异(P=0.901). 结论 IL-11可有效预防小鼠allo-BMT后aGVHD,减轻其症状和病理损害程度,显著延长平均生存时间.
AbstractObjectiveTo evaluate the prophylactic effect of interleukin-11(IL-11) on acute graft-versus-host disease (aGVHD) in a murine allogeneic bone marrow transplantation (allo-BMT) model. MethodsAcute GVHD was induced in male BALB/C(H-2d) mice by total body irradiation (TBI) followed by female allogeneic C57BL/6（H-2b）bone marrow and spleen cells transplantation. The experiment groups were designed as follows: control group, IL-11 ( 500μg/(kg·d) group, cyclosporine A (［CsA, 10mg/(kg·d)］ group. The occurrence of aGVHD was assessed by signs of weight loss, diarrhea, ruffled fur, hunched posture and histologic changes of skin, liver and small intestines. ResultsThe survival time of IL-11 group and CsA group were prolonged as compared with that of the control group (P<0.01). aGVHD signs and histologic changes of IL-11 group and CsA group were milder than those of the control group. ConclusionIL-11 can be effective in prevention for aGVHD after allo-BMT in a murine model.It can decrease the aGVHD signs and histologic changes,and markly prolong mean survival time.
