知识与财富的链接
目的:建立刺梨中具有品质特征的维生素C的含量测定方法,测定不同产地刺梨的维生素C。方法:采用反相高效液相色谱法测定,DiamonsilTM C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%磷酸(5∶95)为流动相,流速1mL·min-1,柱温25℃,检测波长为242 nm;样品不经DTT还原直接测定还原型维生素C;经DTT还原后测定总维生素C。结果:测定还原型维生素C在0.074 1～0.666 9μg,r=0.999 9,平均回收率为102.64%,RSD为1.13%;测定总维生素C在0.050 3～0.804 0μg,r=0.999 9,平均回收率为98.32%,RSD为1.12%。结论:该方法可准确测定刺梨中还原型维生素C和总维生素C的含量。利用HPLC法检测贵州中山海拔、相似生境下的野生刺梨成熟果实发现,维生素C含量差异不大,平均含量在1 142.11 g·L-1。
Objective: To establish a simple,accurate method of HPLC to determine reduced and total vitamin C from Rosa roxburghii. Method: The method was developed with the condition as follows:DiamonsilTM C18 colum(4.6 mm × 250 mm,5 μm), methanol-0.1% phosphoric acid(5 ∶95)as the mobile phase with the speed of 1.0 mL ·min-1 and the detection wavelength was 225 nm. Reduced and total vitamin C was detected before and after DTT reduction respedively. Result : The linear range of determination for reduced vitamin C was 0.074 1~0.666 9 μg (r=0.999 9) with the average recovery of 102.64%, RSD 1.13%, and the linear range of determination for total vitamin C was 0.050 25~0.804 μg(r=0.999 9)with the average recovery of 98.32%, RSD 1.12%. Conclusion : The method was simple, specific, and suitable for the determination of reduced and total vitamin C in R. roxburghii. By using HPLC method to detect the vitamin C contents of R. roxburghii with the similar wildlife habitats and altitudes in Guizhou province, it was found they that the content of vitamin C had no difference, and their average content was 1 142.11 g ·L-1.
