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几种真菌产木质素降解酶的比较研究

几种真菌产木质素降解酶的比较研究

ISSN:1001-9499
2006年第31卷第4期
森林保护及野生动物
杜海萍[1],宋瑞清[2],王钰祺[1] DU Haiping
  1. 东北林业大学,黑龙江,哈尔滨,150040
  2. 东北林业大学,黑龙江,哈尔滨,150040;黑龙江省林业科学院,哈尔滨,150081

通过PDA-Bavendamm平板显色反应、PDA-RB亮蓝显色反应、变色圈试验,获得选择性降解稻草木质素的高酶活菌株:糙皮侧耳、彩绒革盖菌和粗毛栓菌。液态产酶活性试验结果表明,粗毛栓菌是供试菌株中Lac、Mnp、Hcel的高产菌株。粗毛栓菌出现2次产Lac酶高峰,分别为第6天和12天,12天产Lac酶达894U/L;产Mnp酶高峰出现在第6天,达185 U/L;在18天时出现产Hcel酶高峰,产酶达62.7U/L。

Three fungi strains with high enzyme activity on selective degradating lignin of traw were got by methods of PDA-Bavendamm,PDA-RB and color-zone,which are Pleurotus ostreatus,Coriolus versicolor and Trametes gallica.The result of producing enzymes activity showed that Trametes gallica is the fungus with highest enzymes activity of Lac,Mnp and Hcel.It has 2 high-peak of lac zymogenic quantity,one is at 6?d,another is at 12?d which is 894?U/ L;zymogenic high-peak of Mnp is at 6d which is185 U/L;at 18d high-peak of Hcel zymogenic quantity appears,it is 62.7?U/L.

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ISSN:1001-9499
2006年第31卷第4期
森林保护及野生动物

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