High performance liquid chromatography and high performance liquid chromatography- electrospray ionization mass spectrometry were developed for the simultaneous determination of ten sulfonylurea herbicide residues in soybeans. Sulfonylurea herbicides were extracted with acetonitrile, followed by hexane partitioning. After the extract was cleaned up with florisil column, sulfonylurea herbicides were analyzed by HPLC-DAD and HPLC-MS and quantified by external standard method. Sample pre-treatment method, LC and LC-MS analytical conditions were critically examined. The results have indicated that the method developed is easier, faster, sensitive and better purification effect. It has also demonstrated that this multiresidue analysis method can meet the requirements for simultaneous determination of many sulfonylurea herbicides in import & export inspection for soybeans.