用正交试验优化了基因工程菌(E.coli/p WSY)产黑色素培养基的组成。配方为:蛋白胨1%、葡萄糖0.2%、硫酸铵0.5%、磷酸氢二钾0.7%、磷酸二氢钾0.3%、氯化钠0.5%,硫酸镁0.01%,为实现微生物发酵法大规模生产黑色素提供了重要数据。
The suitable medium of engineering bacteria (E.Coli/p WSY) for melanin fermentation obtained by means of orthgonal optimization. Fermentation medium (%): Tryptone 1, Glucose 0.2, (NH 4) 2SO 4 0.5, K 2HPO 4 0.7, KH 2PO 4 0.3, NaCl 0.5, MgSO 4 0.01, pH 7.0. This research provioled the important data for the producation on large scale of melanin fermentation by microbiological fermentation.