知识与财富的链接
[目的]研究调心方有效部位(TX0201)和达纳康(EGb761)对β-淀粉样蛋白25-35(Aβ25-35)所致类老年性痴呆(AD)模型大鼠胆碱能系统作用的异同。[方法]采用双侧杏仁核注射Aβ25-35造成类AD大鼠模型,观察大鼠Morris水迷宫空间记忆能力、乙酰胆碱(AchE)活性,并分别通过逆转录多聚酶链反应(RT-PCR)和免疫组化方法研究类AD大鼠淀粉样前体蛋白(APP)mRNA表达和Aβ阳性神经元。[结果]1)类AD模型大鼠Morris水迷宫测试出现空间记忆能力下降,海马AchE含量降低,皮层、海马APPmRNA的表达上调,Aβ阳性神经元增加;2)TX020和EGb7611可以改善类AD大鼠空间记忆能力;下调海马APPmRNA的表达。[结论]TX0201和EGb761显著改善Aβ诱导的痴呆模型大鼠学习记忆障碍,可能是通过降低海马APPmRNA表达发挥作用。
[Objective] To research the mechanism of TX0201 Active position of Tiaoxin prescription(heart-regulating prescription)and EGb761(Danakang)on cholinergic system in rat with Alzheimer’s disease(AD).[Methods] AD was induced by Ab25-35 injected into the bilateral corpus amygdaloideum to observe the spatial learning and memory ability,AChE activity,and the expression of β-amyloid and β-amyloid precursor protein(APP)by RT-PCR and immunohistochemistry in the brain of the animal.[Results] In rats with AD the spatial learning and memory ability was damaged significantly,AChE activity decreased in its hippocampus.The expression of β-amyloid and APP mRNA increased in the cortex and hippocampus.TX0201 could improve the spatial learning and memory disturbance,decreased the expression of APP mRNA in hippocampus.[Conclusions] The TX0201 and EGb761 may obviously improve the learning and memory handicap of rats with AD by decreasing the expression of APP mRNA in hippocampus.
