A water-soluble cross-linking polymer of β-CD with epichlorohydrin was synthesized. The actions of polymer are different from β-CD when it reacts with the organic guests to form inclusion complexes. The multiunits of β-CD containing in the polymer possessed synergic effect on different guest parts of organic molecules. In this paper the inclusion reactions of polymer with a series of organic analytical reagents containing two guest groups have been studied in detail. The results showed that the polymer provided more specific effects and functions than β-CD on the enhancement of solubility, absorption and fluorescence spectra. It is possible to be a new molecular recognition method for certain organic compound containing two aromatic guest groups.