知识与财富的链接
本文研究了影响毛樱桃(Prunus tomentosa Thunb.)茎尖培养的有关因素。毛樱桃茎尖在 MS,G,L 三种培养基上均可增殖生长,但以在 L 培养基中生长较好。在附加的六类激素中,以 IBA,BA 最为有效,适宜的浓度为 IBA 1.0,BA 1.0或 ZT 1.0。诱导生根以1/2培养基附加 NAA 0.1较好,削伤和暗培养可大大提高生根率。移栽入土前,在0.1%克菌丹溶液中浸根5分钟可提高成活率。
This paper deals with some factors affecting shoot tip culture of Manchu cherry(Prunus tomentosa Thunb.)in vitro.Manchu cherry shoot tips can propagate on such three kinds of mediums as MS,G and L mediums,growing vigorously on L medium.In the additional hormones of six kinds,IBA ana BA are the most effective ones with the optimum concentration of IBA 1.0,BA 1.0 or ZT 1.0.1/2L medium supplemented with NAA 0.1 is better for the induction of shoot propagation.The rooting rate can be greatly improved by a basal wound on one or two sides of the shoot base and incubation in the dark.prior to trans- planting,the roots of plantlets should be soaked in Captan solution with a concentration of 0.1% for 5 minutes so that the survival rate may be increasingly raised.