知识与财富的链接
本文首次将高速逆流色谱法应用于微生物发酵液提取物中辅酶Q10的分离纯化,建立了一套可用于其制备分离的逆流色谱溶剂体系正庚烷－乙睛－二氯甲烷(12：7：3.5, v/v/v)。500mg发酵液粗提物经一步制备分离,可得到绝对纯度在98％以上辅酶Q10130mg。比较表明,该方法较传统的硅胶柱层析和结晶相结合的纯化方法在产物纯度、回收率及产率等方面都有一定的优势。
High-speed counter-current chromatography (HSCCC) is applied for the first time in purification of Coenzyme Q10 (CoQ10) from a fermentation broth extract. A non-aqueous two-phase solvent system composed of heptane-acetonitrile- dichloromethane (12:7:3.5, v/v/v) is selected by analytical HSCCC and used for preparative purification of CoQ10 from 500 mg of the crude extract. The separation yielded 130 mg of CoQ10 at high purity of over 98%. The overall results of the present studies show advantages of HSCCC in the purity, recovery and yield of CoQ10 over the silica gel chromatography with subsequent crystallization.
