知识与财富的链接
目的:建立高效液相色谱法测定岩黄连生物总碱中脱氢卡维丁、盐酸巴马汀及盐酸小檗碱含量的方法,为完善岩黄连生物总碱的质量评价提供依据。方法:采用高效液相色谱法,以Agilent XDB C18(4.6 mm×150 mm,5μm)为色谱柱,以乙腈-0.01 mol.L-1磷酸二氢钾水溶液(18.5∶81.5)为流动相,流速1.0 mL.min-1,柱温30℃,检测波长347 nm。结果:脱氢卡维丁进样量在15.375～92.25 mg.L-1(r=0.999 9)呈良好的线性关系,平均加样回收率96.43%,RSD 1.13%;盐酸巴马汀进样量在14.91～89.46 g.L-1(r=0.999 9)呈良好的线性关系,平均加样回收率96.28%,RSD 1.22%;盐酸小檗碱进样量在3.525～28.2 mg.L-1(r=0.999 7)呈良好的线性关系,平均加样回收率为103.02%,RSD 1.35%。结论:所建立的方法简便、准确、重复性好,可以用于控制岩黄连生物总碱的质量。
Objective: To establish an HPLC method for simultaneously determining dehydrocavidine,palmatine hydrochloride and berberine hydrochloride in total alkaloids of Corydalis saxicola. Method: An Agilent XDB C18 column (4.6 mm×150 mm,5 μm) was used, the acetonitrile-0.01 mol ·L-1 potassium dihydrogen phosphate solution(18.5 ∶81.5)as mobile phase, the column temperature was at 30 ℃, the detection wavelength was set at 347 nm,the flow rate was 1.0 mL ·min-1. Result: The liner range of dehydrocavidine was 15.375-92.25 mg ·L-1 (r=0.999 9,n=6)and the average recovery was 96.43%(RSD 1.13%).The liner range of palmatine hydrochloride was 14.91-89.46 mg ·L-1 (r=0.999 9,n=6) and the average recovery rate was 96.28%(RSD 1.22%).The liner range of berberine hydrochloride was 3.525-28.2 mg ·L-1 (r=0.999 7,n=6).and the average recovery rate was 103.02%(RSD 1.35%). Conclusion: The method appeared to be accurate, sensitive and reliable,and can be used to control the quality of the total alkaloids of C.saxicola.
