知识与财富的链接
采用无血清培养的方法,分析了促肾上腺激素皮质激素(adrenocorticotropic hormone,ACTH)、黄体生成素(luteinizing hormone,LH)、cAMP、内啡肽(endorphin)和纳络酮(naloxone)对原代共培养的恒河猴(Macaca mulatta)睾丸间质细胞与支持细胞雌二醇分泌水平的影响。结果显示:ACTH、LH、cAMP和纳络酮对原代共培养恒河猴睾丸间质细胞与支持细胞的雌二醇分泌水平具有促进作用,并且这种影响与共培养的间质细胞数量呈线性关系,即共培养的间质细胞数量增加,雌二醇分泌水平亦明显上升;而内啡肽对原代共培养恒河猴睾丸间质细胞与支持细胞的雌二醇分泌水平有明显的抑制作用。研究表明,恒河猴睾丸的间质细胞对支持细胞分泌雌二醇具有调节作用。
By using serum-free co-culture of Leydig cells and Sertoli cells isolated from Rhesus Monkey(Macaca mulatta),we analyzed the effect of adrenocorticotropic hormone(ACTH),luteinizing hormone(LH),cAMP,endorphin,and naloxone on the secretion of estradiol. We showed that ACTH,LH,cAMP,and naloxone stimulated estradiol production in co-cultured system and this stimulation was enhanced by increased Leydig cells. We also showed that endorphin inhibited estradiol secretion by Leydig cells and Sertoli cells when a certain amount of Leydig cells were present. Thus,these results suggest the amount of Leydig cells may play an important role in estradiol production in Leydig-Sertoli cells co-culture.
