知识与财富的链接
目的 探讨不同波段电磁辐射对大鼠睾丸支持细胞(Sertoli细胞)性别决定相关基因SOX9和WT1表达的影响。方法 原代分离3周Wistar大鼠的Sertoli细胞,应用WT1免疫细胞化学染色鉴定细胞纯度;Sertoli细胞经平均功率密度为100 mW/cm2的S波段微波和X波段微波及场强6×104 V/m的电磁脉冲辐射4 min。应用实时RT-PCR和Western blot方法,检测SOX9、WT1的mRNA和蛋白质的变化。结果 3种波段电磁辐射后Sertoli细胞SOX9 mRNA和蛋白表达在辐射后6和12 h及辐射后的6和24 h均见不同程度的降低(F=15.20和4.84, P<0.05;F=8.46和7.47,P<0.05);WT1 mRNA和蛋白表达在辐射后6和12 h及辐射后的24 h出现不同程度降低(F=13.46、5.08和10.26,P<0.05)。结论 3种波段电磁辐射后Sertoli细胞性别决定相关基因(SOX9、WT1)表达呈不同程度的降低。Sertoli细胞SOX9、WT1的变化可能参与了电磁辐射致生殖危害的病理生理过程。
Objective To explore the changes of SOX9 and WT1 expressions in rat Sertoli cells irradiated by EMP (electromagnetic pulse), S-HPM (S-band high power microwave) and X-HPM (X-band high power microwave). Methods Primary Sertoli cells were isolated from 3-week-old Wistar rats and its purity was immunocytochemistrically indentified with WT1. After exposure to 6×104 V/m EMP, 100 mW/cm2 S-HPM and X-HPM for 4 min respectively, SOX9 and WT1 expressions in Sertoli cells were determined with real-time PCR and Western blot, respectively. Results SOX9 mRNA expression was decreased at 6 and 12 h post-irradiation of three different bands of electromagnetic microwave (F=15.20 and 4.84, P<0.05). SOX9 protein expression was also decreased at 6 and 24 h after irradiation (F=8.46 and 7.47, P<0.05). WT1 mRNA expression was decreased at 6 and 12 h (F=13.46 and 5.08, P<0.05), but its protein expression was decreased only at 24 h post-irradiation (F=10.26, P<0.05). Conclusions Three bands of electromagnetic radiation reduce the expressions of SOX9 and WT1 in rat Sertoli cells, which may provide molecular foundation for genital system hazards induced by microwave radiation.