知识与财富的链接
作为微纳米科学理论与技术迅猛发展的代表,原子力显微镜(atomic force microscopy,AFM)在其25年的发展过程中极大地推进了生物学在微纳米尺度上的拓展,为微纳米生物学的诞生与发展提供了重要技术手段.本文在介绍AFM基本原理和检测模式的基础上,结合作者在该领域的研究成果和工作经验,从生物结构与形态学研究、表面物化性质表征、生物大分子的力学操纵三方面综述了AFM在细胞与生物大分子超微结构与生物力学特性研究中的具体应用,并重点探讨了AFM在细胞与生物大分子科学研究中亟待改进和解决的科学与技术问题,提出了一些探讨性的见解和建议.
To be the representative fruition resulted from the rapid development in micro-nano theory and technology,atomic force microscopy(AFM) has greatly promoted the expansion of biological research in micro-nano scale,and facilitated the birth and development of micro-nano biology as an important technique in its 25-year evolutional progress.Based on the fundamental principles and detection modes of AFM,as well as the author’s research findings and work experience in this field,the paper reviews the application of AFM in the study on ultrastructure and biomechanical properties of cells and biomacromolecules in the aspects of biological structure and morphology,surface physicochemical characterization and mechanical manipulation of biological macromolecules,and focus on some important scientific and technical problems on AFM in micro-nano biomedical research needed to be improved and solved urgently,with exploratory insights and recommendations for potential users in ultrastructure and biomechanics of cells and biomacromolecules.
