Planthopper is one of the most destructive insect pests of rice. Wild species of rice are a valuable source of resistance genes for developing resistant cultivars. Substitution lines population was effectively used in identification and utilization of favorable genes from wild rice. In the present study a substitution lines population originating from the cross of Oryza sativa and Oryza minuta was screened for obtaining resistant resources to two brown planthopper(BPH) and whitebacked planthopper(WBPH). Among 371 substitution lines, 11 and 7 were identified to be resistant to BPH and WBPH respectively, 5 were identified to be resistant to BPH and WBPH. This is the first report of WBPH resistant materials from Oryza minuta. For genetic analysis of the resistance, 2 resistant substitution lines, named K41 and P114, were crossed with indica rice variety IR24, Gui 99, 1025. And it was found that the plants of F1 from K41 were resistant to BPH and WBPH, but the plants of F1 from P114 were susceptible to the disease. Segregation of resistant and susceptible progenies fitted a 1:3 ratio in two F2 populations derived from P114, while segregation of resistant and susceptible progenies fitted a 9:7 ratio in two F2 populations derived from K41. The results suggested that the resistance originated from resistant source P114 was controlled by one recessive gene, and the resistance originated from resistant source K41 was controlled by two dominant genes interactions. These results here will be useful to breeding and mapping of resistance to BPH and WBPH gene from Oryza minuta.